joint analyses of transcriptomic and metabolomic data to probe ryegrass-endophyte symbiosis
DOI:
https://doi.org/10.33584/rps.13.2006.3051Abstract
Fungal endophytes (Neotyphodium lolii) in perennial ryegrass (Lolium perenne) produce a range of bioactive alkaloids which are implicated in both toxicity to grazing animals and resistance to insects. The understanding of regulatory and biochemical mechanisms of the symbiosis will provide clues for the genetic manipulation of beneficial alkaloid production. This paper presents approaches to analyse data from high-throughput microarray experiments and targeted metabolomic analyses. Combined with bioinformatics analyses, potential genes were found associated with the accumulation of alkaloids and other metabolites. The advantages and limitations of our approach to address the molecular mechanisms of the symbiosis will be discussed. Keywords: Lolium perenne, Neotyphodium lolii, metabolomics, microarray
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