Dual Affymetrix GeneChip analysis of the perennial ryegrass-endophyte symbiosis

Authors

  • L.J. Johnson
  • C.R. Voisey
  • R.D. Johnson
  • A.K. Khan
  • Z.A. Park
  • M. Ramakrishna
  • M. Cao
  • D. Simon
  • M. Christensen
  • G.T. Bryan
  • S. Rasmussen

DOI:

https://doi.org/10.33584/rps.13.2006.3137

Abstract

Grass associations with Epichloë/Neotyphodium endophytes display enhanced fitness as well as prolonged field persistence over their endophyte free equivalents. To gain a comprehensive understanding of the complex biological interactions that occur between a plant host and fungal symbiont, a transcriptomics approach using custom designed Affymetrix GeneChip®s was employed. We are currently comparing and analysing symbiotic interactions of perennial ryegrass (Lolium perenne) with endophytes N. lolii and E. festucae as well as comparing it against endophyte-free perennial ryegrass. Both N. lolii and E. festucae have been grown in culture in order to compare in planta versus in vitro gene expression. Additionally, targeted gene replacements in E. festucae have been performed and a comparative analysis of the knock-outs with wild-type E. festucae infected plants is in progress. These comparative analyses have revealed changes in gene expression which may lead to the identification of gene pathways/networks and the roles of these genes in symbiosis. Keywords: Neotyphodium, Epichloë, transcriptomics, symbiosis

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Published

2007-01-01

How to Cite

Johnson, L., Voisey, C., Johnson, R., Khan, A., Park, Z., Ramakrishna, M., Cao, M., Simon, D., Christensen, M., Bryan, G., & Rasmussen, S. (2007). Dual Affymetrix GeneChip analysis of the perennial ryegrass-endophyte symbiosis. NZGA: Research and Practice Series, 13, 509–513. https://doi.org/10.33584/rps.13.2006.3137

Issue

Vol. 13 (2007): 6th International Symposium on Fungal Endophytes of Grasses

Section

Past volumes

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Copyright

This work is licensed under a Creative Commons Attribution-Non Commercial-NoDerivatives 4.0 International License. Rights granted to the New Zealand Grassland Association through this agreement are non-exclusive. You are free to publish the work(s) elsewhere and no ownership is assumed by the NZGA when storing or curating an electronic version of the work(s). The author(s) will receive no monetary return from the Association for the use of material contained in the manuscript. If I am one of several co-authors, I hereby confirm that I am authorized by my co-authors to grant this Licence as their agent on their behalf. For the avoidance of doubt, this includes the rights to supply the article in electronic and online forms and systems.

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