The role of cAMP signalling in the symbiosis between Epichloe festucae and Lolium perenne

Authors

  • C.R. Voisey
  • M.J. Christensen
  • R.D. Johnson
  • L.J. Johnson
  • A.K. Khan
  • Z.A. Park
  • M. Ramakrishna
  • M. Cao
  • J.P. Koolaard
  • A. Koulman
  • S. Borchert
  • C.N. Gaborit
  • J.M. Pratt
  • S. Rasmussen
  • G.T. Bryan

DOI:

https://doi.org/10.33584/rps.13.2006.3121

Abstract

In fungal pathogenesis the cAMP signalling cascade is usually essential for virulence. Deletion of the adenylate cyclase gene, the enzyme that synthesises cAMP, often results in an attenuated or avirulent phenotype. Our aim was to identify the signalling mechanisms regulating colonisation of perennial ryegrass (Lolium perenne) by the fungal symbiont Epichloë festucae Fl1. We have identified genes from several signalling networks, and here report on the outcomes of targeted disruption of the E. festucae Fl1 adenylate cyclase gene (acyA). A dual genome (endophyte/ ryegrass) Affymetrix GeneChip® has been synthesised and we are undertaking large scale transcript profiling of the L. perenne/ E. festucae ΔacyA symbiotum to identify target genes regulated by the endophyte cAMP signalling network. Keywords: cAMP, adenylate cyclase, acyA, Neotyphodium lolii, Epichloë festucae, symbiosis, Affymetrix GeneChip

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Published

2007-01-01

How to Cite

Voisey, C., Christensen, M., Johnson, R., Johnson, L., Khan, A., Park, Z., Ramakrishna, M., Cao, M., Koolaard, J., Koulman, A., Borchert, S., Gaborit, C., Pratt, J., Rasmussen, S., & Bryan, G. (2007). The role of cAMP signalling in the symbiosis between Epichloe festucae and Lolium perenne. NZGA: Research and Practice Series, 13, 457–459. https://doi.org/10.33584/rps.13.2006.3121

Issue

Vol. 13 (2007): 6th International Symposium on Fungal Endophytes of Grasses

Section

Past volumes

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This work is licensed under a Creative Commons Attribution-Non Commercial-NoDerivatives 4.0 International License. Rights granted to the New Zealand Grassland Association through this agreement are non-exclusive. You are free to publish the work(s) elsewhere and no ownership is assumed by the NZGA when storing or curating an electronic version of the work(s). The author(s) will receive no monetary return from the Association for the use of material contained in the manuscript. If I am one of several co-authors, I hereby confirm that I am authorized by my co-authors to grant this Licence as their agent on their behalf. For the avoidance of doubt, this includes the rights to supply the article in electronic and online forms and systems.

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