Vol. 18 (2025): Proceedings of the 11th International Symposium of Grass Microbial Endophytes

View Vol. 18 (2025): Proceedings of the 11th International Symposium of Grass Microbial Endophytes

Grass endophytic microbes for sustainable agricultural production

Editor: Stuart Card

DOI: https://doi.org/10.33584/rps.18.2025

Published: 2025-12-04

R&P 18 Grass Microbial Endophytes 2025

A historical perspective on the International Symposium of Grass Microbial Endophytes

Carolyn Young, David Hume

11
- PDF
Epichloë endophytes – underpinning sustainable food production from pastoral systems

John Roche

13
- PDF
Epichloë evolution: What ChatGPT won’t tell you

Christopher Schardl

15-16
- PDF
Epichloë endophytes – from science to commercial impact

Linda Johnson

17
- PDF
Prevalence of seedborne symbionts in grass populations: connecting host fitness, endophyte transmission and intergenerational effects

Pedro Gundel

19
- PDF
Coevolution of Pooideae grasses and their Epichloë endophytes within a hologenome framework

Pilar Catalán , Alba Sotomayor-Alge , Íñigo Zabalgogeazcoa , Diana Calderón , Chunlin Chen , Wenjie Mu, Miguel Campos , Ma Angeles Decena , Beatriz Larruy, Ernesto Pérez-Collazos , Luis Ángel Inda

21
- PDF
Decomposition potentialities of Epichloë bromicola from Elymus racemifer

Yuki Hatano, Yuya Maegawa, Natsuko Uenoyama, Takashi Osono

23-28
- PDF
New Zealand’s pastoral ecosystems: challenges, solutions, and the protective role of Epichloë endophytes

Katrin Gabriela Hewitt, Rainer Hofmann, Olivier Ball, Sarah Finch, Racheal Bryant, Alison Popay

29-44
- PDF
Identification of biomarkers associated with the survival of selected Epichloë endophytes while in seed storage

Sushma Prakash, Paul Maclean, Madison Hooper, Min Zhou, Wade Mace, Stuart Card

45-54
- PDF
Using tall fescue symbiotic diversity to improve pasture resilience and sustainability

Alayna Jacobs, Jim Nelson, A. Elizabeth Carlisle, Mahtaab Bagherzadeh, Huihua Ji, Victoria Stanton, Rebecca McCulley

55
- PDF
Mentoring undergraduate research with the Epichloë-tall fescue symbiosis

Tammy Tintjer

57
- PDF
Combined analysis of population genetic structures in a living-fossil grass Brachyelytrum erectum, and its endophyte Epichloë brachyelytri.

Christopher Schardl , Padmaja Nagabhyru , Andrew Tapia , Patrick Calie , Simona Florea , Neil Moore , Jerzy Jaromczyk , Carolyn Young

59
- PDF
Transgenerational trade-off: the hidden costs of maternal defoliation in grass–endophyte symbiosis

Benjamin Fuchs, Anne Muola, Marjo Helander, Kari Saikkonen

61
- PDF
Hidden players in evolution: The impact of mutualistic fungal Epichloë endophytes on niche breadth and diversification across Poa (bluegrass)

Eleanor Hay, Christopher Searcy, Michelle Afkhami

63
- PDF
Enhancing plant defence: The role of mutualistic fungi in silicon-mediated pest resistance

Ximena Cibils-Stewart, Wade Mace, Jeff Powell, Alison Popay, Fernando Lattanzi, Susan Hartley, Scott Johnson

65-66
- PDF
The pyrrolodiazipic acids: a new class of alkaloids produced during the virulent sexual cycle of Epichloë fungi

Daniel Berry, Katrin Grage, Christopher Schardl, Yvonne Becker, Wade Mace, Arvina Ram, Yan-Ni Shi, Carolyn Young, Paul Dijkwel, Artemis Treindl, Adrian Leuchtmann, Barry Scott

67
- PDF
Can you see the light? How microbes and polar light environments orchestrate plant adaptations and global biodiversity

Kari Saikkonen

69
- PDF
Epichloë endophytes mediate transgenerational effects in Achnatherum inebrians by enhancing photosynthetic capacity under drought stress

Chao Xia, Wen He, Xingxu Zhang

71
- PDF
Similarities and differences of metabolic potential among three Epichloë species by LC-MS

Tian Wang, Taixiang Chen, Chunjie Li

73
- PDF
Multi-omics elucidation of the phytohormone biosynthetic pathways in Epichloë sinensis

Yang Luo, Pei Tian

75
- PDF
The dynamics of selfish self-splicing introns in mitochondrial genomes of Epichloë endophytes

Jennie Chan, Mauro Truglio, Christopher Schardl, Murray Cox, Carolyn Young, Austen Ganley

77
- PDF
CRISPR-Cas9 gene insertion in Epichloë species

Taryn Miller, Debbie Hudson, Nazanin Noorifar, Wade Mace, Richard Johnson, Linda Johnson

79-88
- PDF
Decoding horizontal gene transfer mechanisms in Epichloë–grass symbiosis

Haotian Shi, Haijuan Zhang , Chunjie Li, Lei Lei

89
- PDF
Identification of a domain of unknown function family gene involved in mediating the drought tolerance of Achnatherum inebrians colonised by an Epichloë endophyte

Yali He, Haijuan Zhang, Yiyi Wang, Chunjie Li, Lei Lei

91
- PDF
Establishment of an efficient genetic transformation system for the Achnatherum inebrians-Epichloë symbiosis

Zhixia Wang, Yiyi Wang, Jinhao Shao, Lei Lei

93
- PDF
Dissection of the epoxyjanthitrem pathway in Epichloë sp. LpTG-3 strain AR37 by CRISPR gene editing to deliver improved SDN-1 type endophytes for grasses

Linda Johnson, Natasha Forester, Debbie Hudson, Taryn Miller, Wade Mace, David Hume, Pranav Chettri, Alison Popay, Sarah Finch, Paul Maclean, Jaspreet Sidhu, Christine Voisey, Richard Johnson

95
- PDF
Split-marker recombination for fast and efficient targeted deletion of Epichloë genes

Haijuan Zhang, Haotian Shi, Chunjie Li, Lei Lei

97
- PDF
An efficient protoplast transient expression system developed for Achnatherum inebrians

Yiyi Wang, Jinhao Shao, Lei Lei

99
- PDF
The novel host barley produced a rapid defence response to Epichloë endophyte by elucidating gene regulation

Kamran Malik, Taixiang Chen, Chunjie Li

101
- PDF
Receptor-like kinases (RLKs) mediate enhanced immune responses in the Achnatherum inebrians-Epichloë gansuensis symbiotic system against Claviceps pathogen infection

Tao Yang, Haijuan Zhang, Haotian Shi, Lei Lei

103
- PDF
Diversity, ecology and applications of Epichloë endophytes from South America

Leopoldo Iannone

105
- PDF
An Epichloë endophyte associated with the Afromontane grass Festuca simensis

Stuart Card, Michael Christensen, Nicholas Ellison, Marty Faville, David Hume, Richard Johnson, Wade Mace, Mary Namaganda, Jaspreet Singh Sidhu, Wei Zhang, Alan Stewart

107-119
- PDF
Harnessing rhizosphere Bacillus and Epichloë symbionts to promote the performance of Achnatherum inebrians within arid ecosystems

Jinjin Liang, Xingxu Zhang

123
- PDF
How Epichloë and arbuscular mycorrhizal fungi interact with wild barley under salt stress

Yufan Pang, Jinchen Li, Zhenjiang Chen, Chunjie Li

125
- PDF
Uncovering Alkaloid chemotype diversity in natural Festuca–Epichloë festucae symbioses across Iberian ecosystems

Alba Sotomayor-Alge , Beatriz Vázquez de Aldana, Íñigo Zabalgogeazcoa, Padmaja Nagabhyru, Christopher Schardl, Luis Inda, Stephen Lee , Pilar Catalán

127
- PDF
Physiological response of Hordeum brevisubulatum-Epichloë bromicola ecotypes to mixed salt stress

Yurun Zhai, Zhenjiang Chen, Chunjie Li

129
- PDF
Infection frequencies, alkaloid content and characteristics of Epichloë festucae var. lolii in Lolium perenne within German grasslands along a land-use intensity gradient

Jochen Krauss

131
- PDF
Improved methodology for the isolation of Epichloë endophytes from Achnatherum inebrians

Zheng Liang, Xuekai Wei, Chunjie Li

133
- PDF
Occurrence and toxicity of Epichloë endophytes in German grasslands

Tabea Lang, Jochen Krauss

135
- PDF
Exploring wild Bromus-Epichloë interactions in Uruguay: genetic diversity, eco-geographic distribution, and antiherbivore effects

Lucia Meneses, Carolyn Young, Leopoldo Iannone , Ximena Cibils-Stewart, Federico Condón

139
- PDF
Exploring Epichloë endophytes in Scottish wild barley for enhanced crop resilience

Lorena Rangel, Sarah Light, Victoria Buswell, Beatrix Keillor, Damian Bienkowski

141
- PDF
Germplasm survey and collection of Festuca sinensis accessions associated with the endophyte Epichloë sinensis

Pei Tian, Tao Hu, Chao Xia, Jiawen Sun, Yanqun Zhao, Zhongxiang Li, Mei Zhang, Longxuan Cui, Jinhui Shen, Yang Luo, Yue Gao, Wei Zhou, Taixiang Chen, Tian Wang, Mingxiang Du, Wencong Liu, Chunjie Li, Zhibiao Nan

145
- PDF
Fungal endophytes for disease control in cereals

David Collinge, Arianna Petrucci, Alfia Khairullina, Seán Conlan, Sabrina Sarrocco, Hans Jørgensen, Birgit Jensen

147
- PDF
Breeding turfgrasses with Epichloë endophytes for abiotic and biotic stress tolerance

Steve Reid, Barbara Hinds-Cook, Alan Stewart

149
- PDF
AR128 - a new Epichloë fungal endophyte product for ryegrass

John Caradus, David Hume, Alison Popay, Anouck de Bonth, Russell Croy, Natasha Forester, Richard George, Sarah Rosanowski, Paul Maclean, Wade Mace, Marty Faville, Michael Norriss, Brian Maw, Katrin Hewitt, Joanne Jensen, Sarah Finch

151-169
- PDF
Impact of selected Epichloë endophyte on perennial ryegrass yield performance across regions in New Zealand

Justine Ferguson, Wayne Nichol, Allister Moorhead, Sarah McKenzie

171-180
- PDF
Effects of fungicide treatment on transmission of Epichloë endophyte in rye (Secale cereale)

David Hume, Jo Drummond, Phil Rolston, Anouck de Bonth, Paul Maclean, Wayne Simpson, Richard Johnson, John Caradus

181-188
- PDF
Physiological responses and ergot alkaloid kinetics in steers rotating grazing toxic endophyte-infected tall fescue

Ignacio Llada, Jeferson Lourenco, Mikayla, Utsav LAMICHHANE, Matthew Ross, Garret Suen, Dean Jones, Nicholas Hill, Nikolay Filipov
Epichloë is not associated with the pollen of infected ryegrass plants

Natasha Forester, Pranav Chettri, Debbie Hudson, Richard Johnson, Linda Johnson

213-222
- PDF
Epichloë endophyte survival in rye (Secale cereale) and perennial ryegrass (Lolium perenne) seeds under different storage conditions

Anouck de Bonth, Paul Maclean, Richard Johnson, Wayne Simpson, John Caradus, David Hume

223-231
- PDF
Meadow fescue and its Epichloë endophytes in New Zealand

Alan Stewart, David Hume, Fraser Harrison

233-244
- PDF
The impact of selected Epichloë endophytes on invertebrate biodiversity in New Zealand ryegrass sheep pastures

Mark McNeill, Nicole Schon, Russell Croy, Diane Barton, Chikako van Koten, Stephen Goldson, Shengjing Shi

245-259
- PDF
Performance of Sheep Grazing Tall Fescue Cultivars Containing Non-Ergovaline Producing Endophytes

James Thompson, Tom Nichols, Steven Reid

261
- PDF
Exploring ways to improve wheat productivity through the use of beneficial Epichloë endophytes

Lisa Wood, Wayne Simpson, Richard Johnson, Stuart Roy, Rainer Hofmann, John Caradus

263
- PDF
Impact of respiration and CO2 on the survival of Epichloë festucae var. lolii strain AR37 in a cultivar of perennial ryegrass during short-term seed storage

Maria Duter, John Hampton, Rainer Hofmann

265
- PDF
Alliance for Grassland Renewal’s impact on novel Epichloë endophyte tall fescue adoption in the US

Nicholas Hill, Julie Moore

267
- PDF
Temperature-driven variations in Epichloë endophyte-mediated porina resistance in perennial ryegrass

Katrin Hewitt, Joanne Jensen, Jan Sprosen, Chanatda Somchit, Alison Popay

269
- PDF
Impact of AR37, a selected Epichloë endophyte, on Italian ryegrass yield performance across regions in New Zealand

Sarah McKenzie, Justine Ferguson; Wayne Nichol, Allister Moorhead

271
- PDF
Resistance to dollar spot disease in fine fescues is linked to the presence of the endophyte Epichloë festucae

Ruying Wang, Trent Tate, Faith Belanger

275
- PDF
A novel Lolium multiflorum-Epichloë tembladerae association: Evaluation of plant growth, seed production and vertical endophyte transmission under drought and waterlogging conditions

Daniel Ruiz Mínguez , Leopoldo Iannone , Maria Victoria Novas, Natalia Terlizzi , Vanesa Tossi , Patricia Mc Cargo

277
- PDF
Festulolium–Epichloë symbiosis: suppressing stubby root nematodes and delivering nematotoxic plant metabolites

Nyambura Mwangi, Mark Stevens, Alistair Wright, Simon Edwards, Martin Hare, Matthew Back

279
- PDF
Epichloë endophytes from Elymus dahuricus enhance the growth and nutritional quality of Poa pratensis

Mingxiang Du, Chunjie Li, Taixiang Chen

281
- PDF
Perennial ryegrass microbiomes: manipulating microbial communities for enhanced pasture performance

Shengjing Shi, Sandra Young, Emily Gerard, Russell Croy, David Hume, Bernadette Prout, Nigel Bell, Stuart Card, Daniel Bastias, Sandeep Kumar, Hu Wang, Marty Faville, Maureen O’Callaghan

287
- PDF
Effects of Epichloë endophytes on the microbial communities and nutrient concentrations of Achnatherum inebrians roots

Xiumei Nie, Daniel Bastías, Jinjin Liang, Xingxu Zhang

293
- PDF
Using niche occupancy from microbiome data to reveal potential fungal pathogens of established perennial ryegrass roots

Nigel Bell, Bernadette Prout, Faith Mtandavari, David Hume, Sandra Young, Emily Gerard, Lee Alders, Shengjing Shi

295
- PDF
Wild progenitor species of maize (teosintes) have greater seedborne microbial diversity than domesticated maize

Sandeep Kumar, Linda Johnson, Christina Moon, Anna Larking, Jana Schmidt, Qianhe Liu, Richard Johnson

297
- PDF
Arbuscular mycorrhizal fungi coupled with Epichloë endophytes reshaped the rhizosphere microbiome structure and induced synergistic resistance to leaf spot disease in perennial ryegrass

Youlei Shen, Tingyu Duan

299-300
- PDF
Diversity of endophytic fungi in live and dead leaf sheaths of Brachypodium sylvaticum infected with stroma-forming and asymptomatic Epichloë endosymbionts

Yuki Hatono, Shunsuke Matsuoka, Ryunosuke Tateno, Takashi Osono

301
- PDF
Bacterial symbionts of Epichloë fungal endophytes of grasses

Daniel Bastías, Linda Johnson, Stuart Card

303
- PDF
Effects of a fungal volatile organic compound on microbiomes associated with perennial ryegrass and white clover under drought stress

Sereyboth Soth, John Hampton, Steve Wakelin, Hossein Alizadeh, Artemio Mendoza-Mendoza

305
- PDF