Agrobacterium tumefaciens T-DNA mediated transformation of Epichloe festucae
DOI:
https://doi.org/10.33584/rps.13.2006.3125Abstract
Although much is known about the signals and mechanisms that lead to pathogenic interactions between plants and fungi comparatively little is known about fungal-plant mutualistic symbiotic interactions. The association between Epichloë festucae and perennial ryegrass is a powerful experimental system to understand the molecular basis for fungal-plant mutualistic interactions. We have adopted a forward genetics approach to dissect this symbiosis. Previously we described the use of plasmid mutagenesis to isolate a symbiotic mutant. We describe here the development of an Agrobacterium tumefaciens mediated T-DNA transformation system for E. festucae. Keywords: Epichloë festucae, Agrobacterium tumefaciens, TDNA, transformation
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