The analysis of fungal alkaloids in plant fluids

Authors

  • A. Koulman
  • M.J. van Gils
  • G.A. Lane
  • M.J. Christensen
  • K. Fraser
  • B.A. Tapper

DOI:

https://doi.org/10.33584/rps.13.2006.3056

Abstract

Many grasses live in association with fungi (Neotyphodium spp. endophytes) growing in the intercellular spaces of the grass. The endophytes produce a range of alkaloids that protect the grass against grazing animals and insects. There has been speculation about the mobilisation and translocation of different alkaloids. We aimed to determine if metabolites produced by endophytes are mobilised and translocated by the plant. We developed a versatile method for the analysis plant fluids, using a linear ion trap mass spectrometer. Cut leaf fluid and guttation fluid of different grass endophyte associations in ryegrass and tall fescue were analysed. Peramine was detected in the cut leaf and guttation fluid of all ryegrass-endophyte associations that are able to produce peramine, but were found only in the cut leaf fluid of the tall fescue association. Lolines and ergot peptides were also detected in some associations. Keywords: Neotyphodium, ryegrass, tall fescue, guttation, peramine, ergovaline, loline

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Published

2007-01-01

How to Cite

Koulman, A., van Gils, M., Lane, G., Christensen, M., Fraser, K., & Tapper, B. (2007). The analysis of fungal alkaloids in plant fluids. NZGA: Research and Practice Series, 13, 173–175. https://doi.org/10.33584/rps.13.2006.3056

Issue

Vol. 13 (2007): 6th International Symposium on Fungal Endophytes of Grasses

Section

Past volumes

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Copyright

This work is licensed under a Creative Commons Attribution-Non Commercial-NoDerivatives 4.0 International License. Rights granted to the New Zealand Grassland Association through this agreement are non-exclusive. You are free to publish the work(s) elsewhere and no ownership is assumed by the NZGA when storing or curating an electronic version of the work(s). The author(s) will receive no monetary return from the Association for the use of material contained in the manuscript. If I am one of several co-authors, I hereby confirm that I am authorized by my co-authors to grant this Licence as their agent on their behalf. For the avoidance of doubt, this includes the rights to supply the article in electronic and online forms and systems.