Production of haploids and double haploids in annual (Lolium multiflorum) and perennial (L. perenne) ryegrasses

Authors

  • Syed Wajid Hussain
  • Kim Richardson
  • Marty Faville
  • Derek Woodfield

DOI:

https://doi.org/10.33584/rps.12.2006.3048

Abstract

Anthers from 32 genotypes from one line of annual ryegrass (Lolium multiflorum) and 229 genotypes from 15 cultivars of perennial ryegrass (L. perenne) were tested for the production of haploids and double haploids. Six (23%) annual and 71 (31%) perennial ryegrass genotypes produced calli ranging from 1.3-16% and 0.8- 12.2% respectively. However none of the annual ryegrass genotypes produced green plants and only one genotype (Option WH-1) of perennial ryegrass produced 16 (1.8%) green plants. The remaining produced only albino regenerates. Twelve of the 16 green plants were evaluated cytologically for chromosome numbers. Four were haploids thus confirming their origin from haploid pollen. The remaining eight were all diploids. One haploid and one diploid were tested for their homozygous condition using seven simple sequence repeat (SSR) markers and were found to be homozygous at all seven loci. In order to increase the recovery of green regenerates, we have used the responsive genotype Option WH-1 in crosses with three other perennial ryegrass genotypes and the progenies are currently been grown for anther culture

Downloads

Published

2006-01-01

How to Cite

Hussain, S. W., Richardson, K., Faville, M., & Woodfield, D. (2006). Production of haploids and double haploids in annual (Lolium multiflorum) and perennial (L. perenne) ryegrasses. NZGA: Research and Practice Series, 12, 45–48. https://doi.org/10.33584/rps.12.2006.3048

Issue

Vol. 12 (2006): Advances in Plant Breeding

Section

Past volumes

License

Copyright

This work is licensed under a Creative Commons Attribution-Non Commercial-NoDerivatives 4.0 International License. Rights granted to the New Zealand Grassland Association through this agreement are non-exclusive. You are free to publish the work(s) elsewhere and no ownership is assumed by the NZGA when storing or curating an electronic version of the work(s). The author(s) will receive no monetary return from the Association for the use of material contained in the manuscript. If I am one of several co-authors, I hereby confirm that I am authorized by my co-authors to grant this Licence as their agent on their behalf. For the avoidance of doubt, this includes the rights to supply the article in electronic and online forms and systems.

Most read articles by the same author(s)